top of page

Waters Synapt G1

ITEM ID:

4018

ITEM DESCRIPTION

The Waters SYNAPT G1 is designed for leading researchers working at the limits of conventional mass spectrometry capabilities.

Designed for leading researchers working at the limits of conventional mass spectrometry capabilities who need to further characterize and define their samples, Waters SYNAPT™ System offers unique, enabling functionality.

Waters SYNAPT System enables the analysis of samples differentiated by size and shape, as well as mass, to deliver increased specificity and sample definition beyond that achievable by conventional mass spectrometers.

Includes:

• Computer System & Software
• Roughing Pump
• Lockspray ESI

TOF Mass Resolution in Positive Ion
a) V Mode: 10,000 measured on the (M + 6H)^6+ isotope cluster from bovine insulin (m/z 956)
b) W Mode: 17,500 measured on the (M + 6H)^6+ isotope cluster from bovine insulin (m/s 956)

TOF Mass Resolution in Negative Ion
a) V Mode: 10,000 measured on the (M – 4H)^4- isotope cluster from bovine insulin (m/z 1431)
b) W Mode: 17,500 measured on the (M – 4H)^4- isotope cluster from bovine insulin (m/z 1431)

Positive Ion MS Sensitivity
a) V Mode: The peak at m/z 556 from a solution of 50 pg/µL leucine enkephalin in 50/50 acetonitrile/water + 0.1% formic acid, infused at a flow rate of 5 µL/min, will have an intensity of greater than 1,700 counts per second. The instrument will be tuned to 10,000 resolution (as demonstrated on bovine insulin) and the mass range will be set to 1,000 Da.
b) EDC: The peak at m/z 556 from a solution of 10 pg/µL leucine enkephalin in 50/50 acetonitrile/water + 0.1% formic acid, infused at a flow rate of 5 µL/min, will have an intensity of greater than 2,000 counts per second. The instrument will be tuned to 10,000 resolution (as demonstrated on bovine insulin), with sensitivity set to a maximum at 556 Da.

Negative Ion MS Sensitivity:
V Mode: The peak at m/z 503 from a solution of 500 pg/µL raffinose in 70/30 acetonitrile/water (no additives), infused at a flow rate of 5 µL/min, will have an intensity of greater than 1,800 counts per second. The instrument will be tuned to 10,000 resolution (as demonstrated on bovine insulin), and the mass range will be set to 1,000 Da.

Positive Ion MS/MS Sensitivity
V Mode: Using a [Glu]-Fibrinopeptide B solution of 100 fmol/µL, at a flow rate of 5 µL/min and with the instrument tuned for 10,000 resolution (as demonstrated on bovine insulin), the intensity of the most intense y” sequence ion from the MS/MS spectrum of the doubly charged precursor ion (785.8 Da) will be greater than 130 counts per second. This will correspond to a signal-to-noise ratio of greater than 50:1 (after a 3×9 smooth) on the most intense y” sequence ion, for a 1-second scan. The instrument mass range will be set to 2,000 Da.

Negative Ion MS/MS Sensitivity
V Mode: Using a solution of 500 pg/µL raffinose in 70/30 acetonitrile/water, at a flow rate of 5 µL/min and with the instrument tuned for 10,000 resolution (as demonstrated on bovine insulin), the intensity of the fragment ion at 179.1 Da in the MS/MS spectrum of the precursor ion at 503.2 Da will be greater than 130 counts per second. The instrument mass range will be set to 1,000 Da.

Mass Scale Calibration Accuracy
The mass measurement accuracy of the instrument in V-mode, using an internal lockmass, is such that the RMS error between the measured and the accepted masses of peaks which have sufficient intensity, and are free from interference from other masses, will be less than 2 ppm over the range of 150-900 Da.

Mass Measurement Accuracy
The mass measurement accuracy of the instrument, in W-mode, will be better than 2 ppm RMS, based on 10 consecutive repeat measurements of the [M + Na]^+ ion of raffinose (m/z 527.1588), using the [M + H]^+ ion of leucine enkephalic (556.2771) as the LockSpray lockmass. Analyte lockmass peaks must have sufficient intensity and be free of interference from other masses.

Mass Range
The TOF mass range is 20 – 100,000 Da in V-mode, and 20 – 26,500 Da in W-mode.
The quadrupole mass range in non-resolving mode is 20 – 16,000 Da for a 4,000 Da quadrupole, and 20 – 32,000 Da for an 8,000 Da quadrupole.

bottom of page